, IDPs/IDPRs have insertion/deletion prices which are usually higher than those of ordered proteins [45]. All of these observations offer further help for our assertion that disordered regions evolve differently from ordered surface residues and hence that IDPs and IDPRs are disordered in vivo.NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptAcknowledgmentsThis operate was supported in portion by the grant from the “Molecular and Cellular Biology” Plan of the Russian Academy of Sciences (to V.N.U.). C.J.B. was supported by NIH grants 5P20RR16448, 8P20GM103397 for the University of Idaho.AbbreviationsASA CDF CH CTD DBD ER IDP IDPR MHC MoRFs NORS OD PR SAM TAD UPR solvent accessible surface area cumulative distribution function charge-hydropathy C-terminal domain DNA binding domain endoplasmic reticulum intrinsically disordered protein intrinsically disordered protein area key histocompatibility complicated molecular recognition functions no-regular secondary structure oligomerization domain proline-rich area sterile- motif transactivation domain unfolded protein response
J. Funct. Biomater. 2013, four, 74-88; doi:10.3390/jfbOPEN ACCESSJournal ofFunctional BiomaterialsISSN 2079-4983 www.mdpi/journal/jfb/ ArticleIncorporation of Exogenous RGD Peptide and Inter-Species Blending as Techniques for Enhancing Human Corneal Limbal Epithelial Cell Growth on Bombyx mori Silk Fibroin MembranesLaura J. Bray 1,two, Shuko Suzuki 1, Damien G. Harkin 1,2,3 and Traian V. Chirila 1,four,five,6,7,*2 35Queensland Eye Institute, South Brisbane, Queensland 4101, Australia; E-Mail: [email protected] (L.J.B.); [email protected] (S.S.); [email protected] (D.G.H.) Faculty of Wellness, Queensland University of Technologies, Brisbane, Queensland 4001, Australia Institute of Health Biomedical Innovation, Kelvin Grove, Queensland 4059, Australia Faculty of Science and Engineering, Queensland University of Technologies, Brisbane, Queensland 4001, Australia Faculty of Well being Sciences, University of Queensland, Herston, Queensland 4006, Australia Australian Institute of Bioengineering and Nanotechnology, University of Queensland, St. Lucia, Queensland 4072, Australia Faculty of Science, University of Western Australia, Crawley, Western Australia 6009, Australia* Author to whom correspondence need to be addressed; E-Mail: [email protected]; Tel.: +61-7-3010-3368; Fax: +61-7-3010-3390. Received: 22 March 2013; in revised type: six Might 2013 / Accepted: six Could 2013 / Published: 17 MayAbstract: While fibroin isolated from the cocoons of domesticated silkworm Bombyx mori supports development of human corneal limbal epithelial (HLE) cells, the mechanism of cell attachment remains unclear.Cefsulodin Description Within the present study we sought to boost the attachment of HLE cells to membranes of Bombyx mori silk fibroin (BMSF) by way of surface functionalization with an arginine-glycine-aspartic acid (RGD)-containing peptide.(±)-Abscisic acid site Moreover, we’ve examined the response of HLE cells to BMSF when blended using the fibroin developed by a wild silkworm, Antheraea pernyi, which is known to include RGD sequences within its key structure.PMID:27108903 A procedure to isolate A. pernyi silk fibroin (APSF)J. Funct. Biomater. 2013, 4 from the cocoons was established, and blends of the two fibroins were prepared at 5 distinct BMSF/APSF ratios. In an additional experiment, BMSF surface was modified by binding chemically the GRGDSPC peptide employing a water-soluble carbodiimide. Principal HLE have been grown within the.