Ion. Currently, there’s a lack of understanding on the doable involvement of EVs in ZIKV pathogenesis. Our study aims to unravel the part of EVs in ZIKV RNA transmission to the brain, through the BBB. Procedures: Human brain microvascular endothelial cells (HBMEC/D3) were used in our study due to the fact they represent the BBB in vitro. 3 distinct EV isolation strategies (precipitation kit, density gradient and size exclusion chromatography combined together with the density gradient) were performed. Western blot, Transmission electron microscopy and Nanosight tracking analysis confirmed the presence of EVs inside the supernatant of HBMEC/D3 cells. The presence of ZIKV RNA in infected-EVs (IEVs) was evaluated by immunofluorescence and qPCR. Additionally, the impact of IEVs on the BBB was assessed working with a label-free impedance-based biosensor (ECIS, Applied BioPhysics). Results: We confirmed the presence of viral elements in our IEVs, like the NS1 and E proteins of ZIKV. The obtained IEVs have been able to reinfect susceptible cells, even immediately after being pretreated with RNase A. This indicates that the viral RNA resides inside the IEVs. Applying impedance measurements on HBMEC/ D3 cell monolayers, we observed that IEVs, as well as virus control caused comparable and temporal disturbances around the monolayer’s integrity within 30 min post infection. No disturbances were seen upon addition of noninfected EVs. Summary/Conclusion: Our study demonstrates that EVs-derived from ZIKV-infected cells are in a position to PLK1 Formulation transfer proteins and viral RNA to recipient cells. Considering the fact that each IEVs and viral particles can induce comparable modifications on barrier’s integrity it is actually possible that IEVs are involved in an alternative mechanism of ZIKV transmission.OWP2.09=PS02.Deciphering the function of extracellular vesicles on the blood rain barrier during Zika virus infection Antonios Fikatas, Sam Noppen, Peter Vervaeke, Jordi Doijen, Mohammed Benkheil, Christophe Pannecouque and Dominique Schols Laboratory of Virology and Chemotherapy, Rega Institute, KU Leuven, Belgium, BelgiumOWP2.10=PF12.HIV-specific antibody mediated targeting of ENV+ tissues by exosomes Zou Xue, Yuan M’eng, Zheng Nan and Wu Zhiwei Nanjing University, Nanjing, China (People’s Republic)Introduction: The association of Zika virus (ZIKV) with extreme neurological issues has gained enhanced interest over the final decade. Having said that, the mechanism by which ZIKV crosses the blood rain barrier (BBB) and reaches the brain remains to become elucidated. It isIntroduction: Antiretroviral therapy can successfully suppress HIV replication inside the peripheral blood to an undetectable level. On the other hand, efforts to eradicate the latent virus in reservoirs remain a challenge and are a significant obstacle inside the remedy of HIV patients. Exosomes exhibit substantial promise as an endogenous drugISEV2019 ABSTRACT BOOKdelivery nanosystem for delivering drugs to SSTR1 Formulation reservoir tissues offered their unique properties, like low immunogenicity, innate stability, high delivery efficiency and mostly importantly the capability to penetrate solid tissues as a result of their lipophilic properties. Methods: Within this study, we engineered and expressed the ScFv of a higher affinity HIV-specific monoclonal antibody, 10E8, on exosome surface. Exosomes from 293T cells have been loaded with curcumin by way of saponin, with efficient as much as 34 . 10E8ScFv-expressing exosomes (10E8-Exo) showed highly effective targeting of and curcumin delivery to CHO cell that expresses a trimeric gp140 on its surface (ENV+ cells) in vitro as demon.