Ion. At present, there is a lack of expertise on the achievable involvement of EVs in ZIKV pathogenesis. Our study aims to unravel the function of EVs in ZIKV RNA transmission towards the brain, by way of the BBB. Techniques: Human brain microvascular endothelial cells (HBMEC/D3) had been utilised in our study considering the fact that they represent the BBB in vitro. 3 distinctive EV isolation solutions (precipitation kit, density gradient and size exclusion chromatography combined together with the density gradient) were performed. Western blot, Transmission electron microscopy and Nanosight tracking analysis Adiponectin Proteins Gene ID confirmed the presence of EVs within the supernatant of HBMEC/D3 cells. The presence of ZIKV RNA in infected-EVs (IEVs) was evaluated by immunofluorescence and qPCR. Also, the impact of IEVs on the BBB was assessed employing a label-free impedance-based biosensor (ECIS, Applied BioPhysics). Results: We confirmed the presence of viral elements in our IEVs, including the NS1 and E proteins of ZIKV. The obtained IEVs have been capable to reinfect susceptible cells, even following becoming pretreated with RNase A. This indicates that the viral RNA resides inside the IEVs. Using impedance measurements on HBMEC/ D3 cell CD49b/Integrin alpha-2 Proteins MedChemExpress monolayers, we observed that IEVs, at the same time as virus manage triggered equivalent and temporal disturbances around the monolayer’s integrity within 30 min post infection. No disturbances were noticed upon addition of noninfected EVs. Summary/Conclusion: Our study demonstrates that EVs-derived from ZIKV-infected cells are able to transfer proteins and viral RNA to recipient cells. Considering that both IEVs and viral particles can induce equivalent adjustments on barrier’s integrity it can be attainable that IEVs are involved in an alternative mechanism of ZIKV transmission.OWP2.09=PS02.Deciphering the function of extracellular vesicles on the blood rain barrier through Zika virus infection Antonios Fikatas, Sam Noppen, Peter Vervaeke, Jordi Doijen, Mohammed Benkheil, Christophe Pannecouque and Dominique Schols Laboratory of Virology and Chemotherapy, Rega Institute, KU Leuven, Belgium, BelgiumOWP2.10=PF12.HIV-specific antibody mediated targeting of ENV+ tissues by exosomes Zou Xue, Yuan M’eng, Zheng Nan and Wu Zhiwei Nanjing University, Nanjing, China (People’s Republic)Introduction: The association of Zika virus (ZIKV) with extreme neurological problems has gained enhanced interest more than the final decade. Having said that, the mechanism by which ZIKV crosses the blood rain barrier (BBB) and reaches the brain remains to be elucidated. It isIntroduction: Antiretroviral therapy can proficiently suppress HIV replication inside the peripheral blood to an undetectable level. Nonetheless, efforts to eradicate the latent virus in reservoirs remain a challenge and are a significant obstacle in the therapy of HIV sufferers. Exosomes exhibit massive guarantee as an endogenous drugISEV2019 ABSTRACT BOOKdelivery nanosystem for delivering drugs to reservoir tissues offered their special properties, like low immunogenicity, innate stability, higher delivery efficiency and largely importantly the capability to penetrate strong tissues as a consequence of their lipophilic properties. Techniques: Within this study, we engineered and expressed the ScFv of a high affinity HIV-specific monoclonal antibody, 10E8, on exosome surface. Exosomes from 293T cells were loaded with curcumin via saponin, with efficient as much as 34 . 10E8ScFv-expressing exosomes (10E8-Exo) showed hugely efficient targeting of and curcumin delivery to CHO cell that expresses a trimeric gp140 on its surface (ENV+ cells) in vitro as demon.