Have been cleaned5.five cm diameter, 7 the flowering period persisted. At the grain size, sediment corers (n = 2, from fauna cm and sediment withtaken prior to, during and immediately after the sexual reproduction period. Sedi of length) have been artificial seawater (30 psu). Then, the sexual spathes contained in each corer werewere driedrecording the flowering work. ment corers counted for for 72 h at 60 to measure the organic matter content material of the The reproductive phenology of Z. noltei sediment (450 , 4.five h). by analysing sediment (OM) by loss on ignition in 1 g ofin Ria de Aveiro was assessedThen, sediment wa the spathes beneath a dissecting microscope and assigning them a stage of improvement separatedon their morphological traits. In sieve shaker distinctive SIEVING TECHNOL into seven sizes making use of a mechanical this way, five (CISA# stages of sexual primarily based OGIES BA 200 N; t = defined (Table 1, Figure1.two mm), and Benidipine web subsequently classified following reproduction have been 20 min, amplitude = two). Stages I, II and III corresponded towards the the period of flowering formation, gravel (2 IV andvery coarse with all the period of seed sand Wentworth scale [28]: fine though stages mm), V coincided sand (1 mm), coarse formation and maturation. Broken and necrotic spathes have been quantified as abortions. (0.5 mm), medium sand (0.25.5 mm), fine sand (0.125.25 mm), really fine sand (0.063The total variety of spathes collected 0.125 mm) and silt and clay (0.063 mm). in every reproductive stage throughout the study period was calculated to describe the general reproductive phenology of Z. noltei within the Ria de Aveiro lagoon. To test variations amongst meadows within the reproductive phenology, the 2.2. Flowering Work and Reproductive Phenology percentage of spathes in each and every reproductive stage was estimated by meadow and date.To study the flowering effort and also the reproductive phenology of Z. noltei inside the Ria de Aveiro lagoon, seagrass corers (n = 4; 9 cm of diameter, 6.five cm of length) had been randomly collected at 5 dates (June, July, August, September and November) in each meadow a the flowering period persisted. At the laboratory, seagrass corers were cleaned from fauna and sediment with artificial seawater (30 psu). Then, the sexual spathes contained in eachPlants 2021, 10, x FOR PEER REVIEW15 ofPlants 2021, ten,Table 1. Description with the 5 sexual reproductive stages utilised to study the reproductive phenology of Z. noltei in Ria de Aveiro. The morphology of every single reproductive stage is shown in Figure two.4 ofTable 1. Description of the five sexual reproductive stages used to study the reproductive phenology of Z. noltei in Ria de Period Stage Description Aveiro. The morphology of each and every reproductive stage is shown in Figure two. Yellow-green spathe, Cholesteryl sulfate Protocol sheath closed; pistils and stamina are visible, aligned onto I the stem Period Stage Description Pistils (IIa) and/or stamina erected (IIb); designs and stigma and/or anthers are Flowering II Yellow-green spathe, sheath closed; pistils and stamina are visible, aligned onto the stem I outside the sheath Flowering II Pistils (IIa) and/or stamina erected (IIb); styles and stigma and/or anthers are outside the sheath Stigma brown, commence to depart from spathe; often with stamina already detached III III Stigma brown, commence to depart from spathe; frequently with stamina currently detached from the spathe in the spathe IV Green spathe with immature seeds; sheath closed IV Green spathe with immature seeds; sheath closed Seed formation Green/brown spathe, dark brown.