Lson CJ, Emson Computer. Restoration of thalamostriatal projections in rat neostriatal
Lson CJ, Emson Computer. Restoration of thalamostriatal projections in rat neostriatal grafts: electron microscopic analysis. J Comp Neurol. 1991; 303:224. [PubMed: 2005239] Yung KK, Bolam JP, Smith AD, Hersch SM, Ciliax BJ, Levey AI. Immunocytochemical localization of D1 and D2 dopamine receptors within the basal ganglia of your rat: light and electron microscopy. Neuroscience. 1995; 65:70930. [PubMed: 7609871]NIH-PA Bim web Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptJ Comp Neurol. Author manuscript; available in PMC 2014 August 25.Lei et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 1.Low energy (A,B) and 40light microscopic views (C,D) of VGLUT1 (A,C) and VGLUT2 (B,D) immunolabeling of rat brain. Pictures A and B present views at a coronal level anterior towards the anterior commissure; note the presence of VGLUT2 thalamostriatal terminals in cortical layer 4 in image B and also the conspicuous absence of VGLUT1 cortical terminals in cortical layer 4 in image A. The 40views reveal the presence of numerous varicosities in striatum immunolabeled for VGLUT1 (C) and VGLUT2 (D), using the varicosities a lot more than twice as abundant for VGLUT1 as VGLUT2.J Comp Neurol. Author manuscript; readily available in PMC 2014 August 25.Lei et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 2.CLSM Cereblon Species Images in (A,C,E) present a single field from a double-labeled section, displaying the total colocalization of immunolabeling in rat striatum for guinea pig (GP) antiVGLUT2 (A) and rabbit (Rb) anti-VGLUT2 (C), as shown by the total labeling overlap in the merged image (E) for (A,C). Conversely, CLSM photos in (B,D,F) present a single field from a double-labeled section, showing the close to absence of colocalization of immunolabeling in rat striatum for guinea pig (GP) anti-VGLUT1 (B) and rabbit (Rb) antiVGLUT2 (D), as shown by the meager overlap inside the merged image (F) for (B,D).J Comp Neurol. Author manuscript; obtainable in PMC 2014 August 25.Lei et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 3.Detail of each and every CLSM image shown in Figure 2. Images in (A,C,E) present magnified views on the upper left from Fig. 2A,C,E, respectively. Similarly, photos (B,D,F) present magnified views in the upper left from Fig. 2B,D,F, respectively. These magnified views make it a lot more feasible to resolve individual terminals, and thereby confirm: 1) the full colocalization observed in rat striatum for guinea pig (GP) anti-VGLUT2 (A) and rabbit (Rb) anti-VGLUT2 (C), as further evidenced by the full labeling overlap in the merged image (E) for (A,C); and two) the near absence of colocalization in rat striatum for guinea pigJ Comp Neurol. Author manuscript; obtainable in PMC 2014 August 25.Lei et al.Pageanti-VGLUT1 (B) and rabbit anti-VGLUT2 (D), as shown by the absence of evident overlap inside the merged image (F) for (B,D).NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptJ Comp Neurol. Author manuscript; obtainable in PMC 2014 August 25.Lei et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 4.CLSM views of immunofluorescence for VGLUT1 (A) or VGLUT2 (B) in fields with fluorescent PHAL labeling of corticostriatal axons and terminals (C,D). Note that corticostriatal terminals in (C) immunolabel for VGLUT1 but those corticostriatal terminals in (D) don’t immunolabel for VGLUT2. This could be seen additional clearly inside the mer.