Bfl-1 Species delivering the very best sustained viral response amongst other combinations of directacting antiviral agents (DAAAs) by attaining 99 sustained viral response decompensated cirrhotic sufferers in a 12 week regimen.two,16 However, using molecular docking analyses,9 Epclusa has shown to exhibit high efficiency against COVID-19.6,7 This was ascribed to the similarity among nonstructural protein 12 (nsp12) in each HCV and SARSCOV-2 RNA polymerases, which was found to possess high potential for SARS-COV-2 viral infection.6,2021 The Authors. Published by American Chemical SocietyThe necessity to monitor such a life-saving drug emerges in the truth that it really is extensively metabolized by liver cytochrome P450 enzymes; CYP2B6, CYP2C8, and CYP3A4.17,18 This makes its metabolism prone to alteration by other inhibiting therapeutic agents that affect those enzymes. To this finish, VLP determination inside the presence of sofosbuvir was reported making use of a plethora of methods, including LC-MS/MS,19,20 UPLC-ESCI MS/MS,21 HPLC-MS/MS,22 and HPTLC,23 in biological fluids and clinical studies. Also, the determination of the two drugs in their pharmaceutical formula was addressed using UPLC,24 spectrofluorimetry,25 UPLC/spectrofluorimetry,26 and spectrophotometry.27 Having said that, the determination range is somewhat pricey and time-consuming. Within this regard, electrochemistry can deliver highly sensitive and speedy analysis procedures which are very simple and cost-effective.28-33 The electrochemical determination of VLP employing the molecularReceived: August 19, 2021 Accepted: September 17, 2021 Published: September 30,doi.org/10.1021/acsomega.1c04525 ACS Omega 2021, 6, 26791-ACS Omegahttp://pubs.acs.org/journal/acsodfArticleFigure 1. Schematic of the preparation of 5-BSA=N-MIL-53(Al) plus the suggested oxidation mechanism of VLP.Figure 2. FESEM photos of (a) NH2-MIL-53 (Al) and (b) 5-BSA=N-MIL-53(Al), (c) XRD of both NH2-MIL-53(Al) and 5-BSA=N-MIL-53(Al), and (d) BET adsorption-desorption isotherms of NH2-MIL-53(Al) and 5-BSA=N-MIL-53(Al).imprinted polymer deposited on a 3D starfish-like hollow nickel skeleton decorated using the MWCNT-Au NP-modified glassy carbon electrode has recently been reported in pharmaceutical formula and biological fluids.34 Despite the accomplished fantastic linearity (5.66-90.60 nM) and low limit of detection (LOD) (0.227 nM), the higher expense from the composite along with the tedious synthesis technique render the entire process hard to adopt for routine analysis.34 These limitations necessitate the search for option platforms. Herein, we demonstrate the use of 5-bromo-salicylaldehydeNH2-MIL-53(Al) (5-BSA) as an efficient and novel metal- organic framework (MOF) material with superior electrochemical properties. Note that NH2-MIL-53(Al) was shown tobe effective within the detection of mercury ions in water having a low detection limit.35,36 Our modification of NH2-MIL-53(Al) yielded a a lot more very K-Ras Molecular Weight responsive MOF with superior conductivity. Especially, the fabricated 5-BSA=N-MIL-53(Al) was investigated as a sensor material for the electrochemical determination of VLP, knowing that sofosbuvir is electrochemically inactive,37,38 in buffer solutions and biological fluids. Our proposed platform presents a low cost, feasible, and hassle-free system of preparing a sensor with the wide variety of linearity (0.11-25.97 M), although delivering a comparable LOD of 8.776 nM.doi.org/10.1021/acsomega.1c04525 ACS Omega 2021, 6, 26791-ACS Omegahttp://pubs.acs.org/journal/acsodfArtic