Cer Society (KWF Grants UM 2012-5506 and 2015-7735 to K.R.), worldwide cancer analysis fund 16-0265 (to K.R.).Procedures: High-grade bladder cancer cell line, UMUC-3, with miR-146a overexpression was established and orthotropically transplanted in SCID mice.Tumour size was measured by in vivo imaging and immunohistochemical analysis was performed to evaluate Jagged-2 Proteins supplier angiogenesis within the tumour. Cellular proliferation, migration and invasion were assessed in human umbilical vein cell (HUVEC) following the addition of EVs from BCa. Uptake of EVs from BCa was also evaluated by PKH67-lavelled EVs. Benefits: Urinary miR-146a-5p level was larger in patients with highgrade BCa and in correlation with all the depths of invasiveness. Tumours generated by UMUC-3-luc miR-146a o/e cells transplanted in mice presented fast tumour development with angiogenesis. We found that the cell proliferation of HUVEC was considerably improved both below transfection with miR-146a mimic and treatment with miR-146a-5penriched EVs. This proliferation along with the uptake of EVs efficiency by HUVEC had been in harmony with the depth of invasiveness of BCa. Summary/conclusion: Our findings indicate that EVs containing miR146a-5p from BCa promoted the proliferation of endothelial cells in tumour. These final results demonstrate that miRNAs in physique fluid, even within EVs, which has been broadly reported for diagnosis, could contribute for the cancer progression. Consequently, miRNAs in EVs is going to be not merely a target molecule for diagnosis but in addition for therapy. Funding: Grand-in-Aid from the Project for Cancer Research and Therapeutic Evolution (P-CREATE) in the Japan Agency for Medical Investigation and Development (AMED).PT04.Exosomes secreted by human osteosarcoma cells market angiogenesis Francesca Perut1; Laura Roncuzzi1; Annamaria Massa1; Elena Torreggiani1; Nicoletta Zini2; Nicola Baldini1 Orthopaedic Pathophysiology and Regenerative Medicine Unit, Rizzoli Orthopaedic Institute, Bologna, Italy; 2CNR – National Investigation Council, Institute of Molecular Genetics, Bologna, ItalyPT04.miRNA-146a-5p in extracellular vesicles facilitate the angiogenesis in high-grade bladder cancer Marta Prieto Vila1; Wataru Usuba1; Nobuyoshi Kosaka2; Fumitaka Takeshita3; Hideo Sasaki4; Tatsuya Chikaraishi4; Takahiro Ochiya1 Division of Molecular and Cellular Medicine, National Cancer Center Study Institute, Tokyo, Japan; 2Division of Molecular and Cellular Medicine, National Cancer Center Study Institute, Chuo-ku, Japan; 3 Basic Revolutionary Oncology Core Center, National Cancer Center Analysis Institute, Chuo-ku, Japan; 4Department of Urology, St. Marianna University College of Medicine, Kawasaki, JapanBackground: Higher recurrence is definitely an everlasting difficulty in bladder cancer (BCa).The classical strategy for detecting recurrence in BCa is cystoscopy, which is a very invasive method. Hence, novel procedures with higher reliability and low-invasiveness are required. To LILRA6 Proteins manufacturer overcome this problem, biomarkers within the urine like microRNA (miRNA) in extracellular vesicles (EVs) are proposed. While the usefulness of miRNA in physique fluid from cancer individuals has been reported, their contribution to the cancer progression has not been shown clearly. Considering the fact that we previously detected higher urinary levels of miR-146a-5p in sufferers with BCa, in this study, we additional analysed the function of miRNA-146a-5p in EVs from BCa.Background: Angiogenesis is often a pivotal method in osteosarcoma (OS) development and progression. Angiogenesis includes a number o.