S and 22 andISEV2019 ABSTRACT BOOKseparated into two distinct groups. Each and every orthologous group was annotated with gene symbols, GO terms, also as functional interactions. Regularly detected orthologous groups were associated with mainly membrane-associated compartments. The GSEA CD73 Proteins Source analysis showed some popular and particular proteins to prokaryote or eukaryote in the categories of biological approach and cellular component. The correlation network evaluation clearly offered a domain-specific terms for example intracellular organelle cilium, cytoplasm ribosome, and ribosome proteasome complicated for eukaryotes, and cytoplasm CD191/CCR1 Proteins Species envelope, extracellular exosome and cell outer membrane for prokayrotes. Summary/Conclusion: Our comprehensive EV proteome analysis could give a functional modules associated with characteristic biological mechanisms in prokayrotes and eukaryotes. This analytical tactic will also deliver a new integrative system to investigate EV proteins and propose an evolutionary protein repertoire of EV.trypsin therapy, we classified the vesicular proteins into 363 candidate real-vesicular proteins and 151 contaminated extravesicular proteins. Protein interaction network analyses showed that candidate real-vesicular proteome is composed of proteins derived from plasma membrane (46.eight), cytosol (36.six), cytoskeleton (8.0) and extracellular area (2.5). However, the majority of the identified proteins derived from other cellular organelles such as nucleus, Golgi apparatus, endoplasmic reticulum and mitochondria were considered as the contaminated extravesicular proteins. Moreover, protein complexes, such as ribosome and T-complex proteins, have been classified as the contaminated extravesicular proteins. Summary/Conclusion: Taken with each other, this trypsin therapy to EVs with large-scale quantitative proteomics enables the evaluation with the real-vesicular proteins in isolated EVs also because the sub-vesicular localization of identified proteins. For that reason, our final results offer the applicable strategy to determine the dependable diagnostic markers of EVs.PF12.Quantitative proteomic evaluation of trypsin-treated extracellular vesicles to evaluate the real-vesicular proteins Gyeongyun Goa, Dong-Sic Choia, Dae-Kyum Kima, Jaewook Leea and Yong Song Ghoba Division of Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea; bDepartment of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of KoreaPF12.Characterization of sweat extracellular vesicles Genevieve Barta, Anatoliy Samoylenkoa, Daniel Fischerb, Anna Kaisanlahtic, Artem Zhyvolozhnyia, Marko Suokasd, Prateek Singha, Justus Reunanenc and Seppo Vainiod University of Oulu, Biocenter Oulu, Laboratory of developmental Biology, Oulu, Finland; bNatural Resources Institute Finland (Luke), Animal Genomics, Jokioinen, Finland; cUniversity of Oulu, Biocenter Oulu, Cancer and Translational Medicine Investigation Unit, Oulu, Finland; dUniversity of Oulu, Biocenter Oulu, Department of Biology, Oulu, Finland; eUniversity of Oulu, Biocenter Oulu, Laboratory of Developmental Biology, Oulu, FinlandaIntroduction: Extracellular vesicles (EVs) are nanosized vesicles surrounded by a lipid bilayer and released in to the extracellular milieu by most of cells. Up to date, various isolation strategies of EVs have been established. Nevertheless, a lot of the present strategies isolate EVs using the contaminated extravesicular proteins, that are co-isolated proteins or non-spec.