Hen these very same mice were immunized with Alum five weeks just after reconstitution, they became extremely sick inside eight days such that the experiment had to be terminated prematurely. Chimeras created from Ndfip1+/+ bone marrow remained healthy even after they were immunized with OVA + Alum and they showed no signs of inflammation in either their skin or lung (Figures S2A and S2B). In contrast, chimeras created from Ndfip1-/- bone marrow that received OVA + Alum had visible lesions on their skin (information not shown) and inflammation inside the skin and lungs (Figures S2C and S2D). T cells from all groups had been analyzed eight days immediately after antigen stimulation for cytokine production. Very few T cells from Ndfip1+/+ bone marrow chimeras made IL-4 in IL-1 Receptor Accessory Proteins Storage & Stability response to in vitro challenge with antigen (Figure 6A). This was true no matter the adjuvant applied and is possibly as a result of the short duration of the experiment. In contrast, the Ndfip1+/+ cells had been capable to produce IFN- in response to ex vivo antigen exposure but only if they had come from animals immunized with OVA + CFA, in line using the recognized Th1-polarizing capacity of this adjuvant. In contrast to these benefits, T cells lacking