Ltration. EVs were characterized by Raman spectroscopy working with a 532 nm laser and the spectra analysed by multivariate analysis. Gold nanoparticles conjugated with antibodies against plasmaSaturday, 05 Maymembrane proteins present on the EVs were employed to detect the EVs using SERS. Final results: The EV spectra show characteristic bands for proteins, lipids and nucleic acids. Multivariate analysis shows a detectable difference between EV populations. SERS enables a lot more targeted detection based on specific proteins present on the EV surface, permitting phenotyping analysis from the population. Summary/Conclusion: This study shows that Raman spectroscopy is usually a beneficial technique for characterization of EVs inside a label-free manner. Additional, SERS can be applied for targeted analysis of your EVs by conjugating antibodies for the metal nanoparticles, with facile multiplexing. Funding: This study was funded by the EPSRC and MRC below grant number EP/L019559/1 (OPTIMA) and by the University of Edinburgh College of Medicine and Veterinary Medicine.PS08.Electrochemical and optical biosensing for the detection of cancer exosomes from breast cancer cells Silio Lima Moura; MercMart Maria Isabel Pividori Grup de Sensors i Biosensors, Departament de Qu ica, Universitat Aut oma de Barcelona, Barcelona, Spaincomposition and functions. Quantification of low concentrations of specific exosomes present in very tiny volumes of clinical samples might cause non-invasive cancer diagnosis and prognosis. Techniques: Employing Carbonic Anhydrase 13 (CA-XIII) Proteins Biological Activity droplet microfluidics, we encapsulated single exosome complexes tagged with an ADAM12 Proteins Molecular Weight enzymatic reporter that produces fluorescent signal for detection. Benefits: Our droplet primarily based single exosome counting immunoassays (droplet digital ExoELISA) strategy enables absolute counting of cancer-specific exosomes to attain unprecedented accuracy. Utilizing a plasma sample of ten , we have been capable to detect as few as five enzymelabelled exosome complexes ( 10-17 M). We demonstrated the application on the droplet digital ExoELISA platform in quantitative detection of exosomes straight in plasma samples from breast cancer patients. Summary/Conclusion: We think our strategy may perhaps possess the potential for cancer early diagnostics and accelerate the discovery of clinical diagnostic cancer exosomal biomarkers.PS08.Virtual Biorepository (VBR): a web-based service for sharing biofluid-, tissue-, cell- as well as other bio-samples Neethu Shah1; Sameer Paithankar1; William Thistlethwaite1; Jorge Arango2; Yashar Kalani3; Julie Saugstad4; Theresa Lusardi4; Joseph Quinn4; Lori Chase5; Tushar Patel5; Andrew R. Jackson6; Sai Lakshmi Subramanian6; Matthew Roth6; Bob Carter7; Fred Hochberg8; Aleksandar Milosavljevic6 Baylor College of Medicine, Houston, USA; 2Phoenix Children’s Hospital, Phoenix, USA; 3Barrow Neurological Institute, Phoenix, USA; 4Oregon Well being Science University, Portland, USA; 5Mayo Clinic, Jacksonville, USA; 6Department of Molecular Human Genetics, Baylor College of Medicine, Houston, USA; 7Department of Neurosurgery, Massachusetts Common Hospital, Boston, MA, Boston, USA; 8UC San Diego, San Diego, USABackground: The identification of novel biomarkers represents a worldwide challenge not merely for the improvement of early diagnostics, but also for patient monitoring and for the evaluation of the efficiency of a therapeutic technique. Exosomes are nano-sized and cup-shaped vesicles, that are currently under intensive study as possible diagnostic biomarkers for many well being disorders, like c.