Nuscript Author Manuscript Author Manuscript2.3.four.7.1.three.2 Flow cytometric detection of cell death in human granulocytes: Human IL27RA Proteins site granulocytes can quickly be obtained by way of density gradient centrifugation of human blood. Quite a few various protocols happen to be published, with some involving dextran sedimentation of RBCs. The protocol we describe here omits the lengthy dextran sedimentation step without affecting the purity of the granulocyte fraction. 1. A total of 20 mL of anti-coagulated blood is diluted with 15 mL PBS and gently layered on best of 15 mL Lymphoflot. Cells are separated by way of centrifugation at 300 g for 30 min devoid of break. The granulocytes layer directly on prime with the RBCs (whitish veil) and are collected and