N blocking buffer in 1:100, 1:500, and 1:100 dilutions, respectively. After a gentle wash, the
N blocking buffer in 1:100, 1:500, and 1:100 dilutions, respectively. After a gentle wash, the cells were incubated with species- or isotype-specific, AlexaFluor-conjugated secondary antibodies (Life Technologies/Thermo Fisher Scientific) diluted…