Minutes. The supernatant was discarded and the pellet resuspended in buffer A (50 mM Tris,
Minutes. The supernatant was discarded and the pellet resuspended in buffer A (50 mM Tris, 2 mM EDTA, five mM MgCl2 at pH 7.0) and incubated at 37 for ten…
Minutes. The supernatant was discarded plus the pellet resuspended in buffer A (50 mM Tris,
Minutes. The supernatant was discarded plus the pellet resuspended in buffer A (50 mM Tris, 2 mM EDTA, 5 mM MgCl2 at pH 7.0) and incubated at 37 for ten…