iparum was used for apicoplast immunofluorescence. The immunofluorescence protocol was similar to that reported with a few minor changes. A solution of 4% paraformaldehyde and 0.1% Triton X-100 was used…
ssary to reliably define hit compounds in the context of a highthroughput screen using the assay conditions reported in this study. Notably, TFEB overexpression resulted in a 3-fold increase in…
iate Rag2 dependent recombination and Rag2 expression is highest in small resting DPs as seen in pre-T LBL. Indeed, Fli-1 binding sites have been found in the Notch1 promoter in…
ence reporting measurements of residual activity of three of the alleles tested. The NAG cell activity assay resulted in average readouts of 24686342 FU for wild-type fibroblasts and 131628 FU…
eparations were incubated with 0.1% toluidine blue at 37uC for 30 min. Cultures were washed extensively with distilled water and photographed. Study Design The CEPs used in this study was…
from spleen cells of 7 independent MRL+/+ and MRL/lpr mice. We found that the more responsive allele of P2X7R, the P451 isoform, is carried by the MRL genetic background and…
nse labeling of distinct populations of interneurons in contrast to only low to moderate levels of labeling in principal neurons like pyramidal cells. In the hippocampal formation, Arg and ADC…
no endothelial cell apoptosis. As mentioned previously, our study only examines one time point. It is possible that apoptosis occurred at an earlier time point than our assessment at the…
same time period. By contrast, 20567609 cells that were colonized with L. crispatus shifted their modes of cell division frequency between 7 and 11 hours; at those times, these cells…
of cells undergoing 21513884 apoptosis was determined. Mitochondrial Membrane Potential Measurements Mitochondrial energization was determined by the retention of JC-1dye. Briefly, MM cells were loaded with JC-1dye during the last…