Cells were subsequently harvested and lysed in 100 ml of 16 cell lysis buffer on ice for 30 min
or 10 min, followed by 55 cycles consisting of two steps: denaturation at 95uC for 30 s, annealing and extension at 60uC for 60 s and then a final cycle…
Recent studies have revealed that phosphorylation of Ser-15 residues of p53 exhibit growth retardation in melanoma
to 200 ml of the Annexin V-binding buffer. After the samples were stained with 2 ml of FITC-labeled Annexin V and 2 ml of PI, the samples were immediately analyzed…