The sequence of purified protease was determined by automated Edman degradation using a Perkin Elmer protein sequencer.Sodium dodecyl sulphate-polyacrylamide gel electrophoresis
The precipitated proteins ended up suspended in Tris-HCl 20 mM pH 7.five, ,025 mM NaCl, and dialyzed for 24 h at four from repeated alterations in the exact same buffer…