The samples were iceincubated for 20 min, mixed with 56 SDS sample buffer, and boiled.Cells were washed with ice-cold PBS and resuspended in hypotonic buffer (10 mM HEPES, pH 7.9, 1.5 mM MgCl2, 10 mM KCl)

The ensuing pellets (i.e., the Triton X-100insoluble fractions) had been sonicated for 10 sec at twenty five% Amp, which generates the insoluble supernatants. The samples had been iceDanshensu (sodium salt)…