As mono-ubiquitination does not direct to protein degradation and can be reverted by deubiquitinating enzymes (DUBs), we screened a total of 70 DUBs for their capability to inhibit Ub-PDK1 upon overexpression (Figure 4A)
PDK1 shuttles between the nucleus and the cytoplasm in a CRM1-dependent and expansion aspect controlled way but the useful importance of this remains unclear . As monoubiquitination has been documented…