After 20 minutes at room temperature, the endogenous peroxidase exercise was inhibited with peroxidaseblocking answer (Dako Authentic, Denmark)
The rabbit polyclonal anti-Hakai antibody (Hakai-2498) and the pEGFP-Hakai assemble had been kindly furnished by Dr. Yasuyuki Fujita . Antibody anti-AKT2 was from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-GFP…